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PDF(834 KB)
血管性痴呆小鼠海马线粒体复合体活性变化
Changes of mitochondrial complex activity in vascular dementia mice
目的:探讨血管性痴呆(VD)小鼠海马线粒体复合体活性变化。方法:抽取并回输约40%总血量加双侧颈总动脉夹闭20 min建立VD模型。病理学研究检测神经元损伤;分光光度计法检测丙二醛(MDA)含量、锰超氧化物歧化酶(Mn-SOD)活性和线粒体复合体活性;Western-blot检测Mn-SOD蛋白表达;Morris水迷宫评估学习记忆能力。结果:与假手术组相比,模型组学习记忆能力显著降低(P<0.05),MDA含量显著升高(P<0.05),Mn-SOD活性和蛋白表达显著降低(P<0.05);与假手术组相比,术后5 d模型组线粒体复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ活性显著降低(P<0.05),术后15,30 d和60 d模型组线粒体复合体Ⅰ、Ⅱ活性无显著变化(P>0.05),复合体Ⅲ、Ⅳ活性显著降低(P<0.05);病理学检测显示模型组海马出现进行性神经元损伤。结论:线粒体复合体III、IV活性异常可能是VD的发病机制之一。
OBJECTIVE To investigate changes of mitochondrial complex activity in vascular dementia (VD) mice. METHODS VD model was established through drawing out and reperfusing 40% of whole blood volume plus clamping carotid arteries of mice for 20 min. Histological observation was performed to evaluate neural damage. Malonaldehyde (MDA), manganese superoxide dismutase (Mn-SOD) and mitochondrial complex activity were determined by using spectrophotometer. Expression of Mn-SOD was tested by western blot, learning and remembering ability were determined by Morris water maze. RESULTS Compared with sham operation group, MDA level was increased in I/R group (P<0.05). Activity and expression of Mn-SOD, learning and remembering ability were decreased in mice in model group (P<0.05). Activities of mitochondrial complex Ⅰ,Ⅱ, Ⅲ and Ⅳ in model group were lower than those in sham operation group in the 5 th day (P<0.05). In the 15th, 30 th and 60 th days, activities of complex Ⅲ and Ⅳ of model group were lower than those in sham operation group (P<0.05), but no significant difference was observed between model group and sham operation group in complex Ⅰ and Ⅱ activities (P>0.05). Progressive neural damages were observed in model group. CONCLUSION Abnormal activities of mitochondrial complex Ⅲ and Ⅳ may be involved in mechanisms of VD.
血管性痴呆 / 记忆 / 线粒体 / 锰超氧化物歧化酶 {{custom_keyword}} /
vascular dementia / memory / mitochondria / Mn-SOD {{custom_keyword}} /
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国家自然科学基金资助项目(编号:81070972)
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